Diagnosis of Aspergillus fumigatus endophthalmitis from formalin fixed paraffin-embedded tissue by polymerase chain reaction-based restriction fragment length polymorphism

摘要

New molecular biological technique of Polymerase Chain Reaction(PCR) based Restriction Fragment Length Polymorphism (RFLP)can identify the species from paraffin-embedded tissue section.We demonstrated Aspergillus fumigatus fungus by PCR-basedRFLP technique from paraffin section of an eyeball of an eight-month-old child removed for endogenous endophthalmitis.